In vivo Fluorometric Measurement of Cerebral Oxidative Stress Using 2'-7' Dichlorofluorescein (DCF).

Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader.

Oxidative stress (OS) has been implicated in various degenerative diseases in aging. In an attempt to quantify OS in a cell model, we examined OS induced by incubating for 30 min with various free radical generators in PC12 cells by using the dichlorofluorescein (DCF) assay, modified for use by a fluorescent microplate reader. The nonfluorescent fluorescin derivatives (dichlorofluorescin, DCFH)...

Fluorophotometric quantitation of oxidative stress in the retina in vivo.

PURPOSE To establish a new fluorophotometric method to quantitate oxidative stress in the retina in vivo with a hydrogen peroxide (H2O2)-sensitive fluorescent dye. METHODS For in vitro fluorophotometric study, nonfluorescent 2',7'-dichlorofluorescein (DCFH) was incubated with H2O2 (10 pM to 100 nM), and the production of fluorescent 2',7'-dichlorofluorescein (DCF) was measured with fluorophot...

Direct in vivo measurement of human cerebral GABA concentration using MEGA-editing at 7 Tesla.

Spectral editing of the GABA spin system is hampered by coediting of macromolecule (MM) coherences. To reduce contamination arising from MMs in spectra edited for GABA, the highest field strength currently available for human experimentation (7 Tesla) and MEGA-based editing were used. Despite judicious choice of experimental parameters, MM contamination was found to arise from field drifts. Whe...

The antioxidant Trolox enhances the oxidation of 2',7'-dichlorofluorescin to 2',7'-dichlorofluorescein.

The use of antioxidants to prevent intracellular free radical damage is an area currently attracting considerable research interest. The compound 2',7'-dichlorofluorescin diacetate (DCFH-DA) is a probe for intracellular peroxide formation commonly used in such studies. During our studies we unexpectedly found that incubation of Trolox, a water soluble vitamin E analog, with DCFH-DA in cell-free...

The measurement of oxidative stress